spec protein ladders for western blotting

#Spec protein ladders for western blotting serial#
#Spec protein ladders for western blotting software#

#Spec protein ladders for western blotting software#

Although Image Studio Software is no longer in development, we continue to sell and support it. Image Studio™ Softwareįor instructions on entering marker molecular weights and sizing bands in Image Studio Software, visit /chameleon-sizing. For more information, see the instructions for the Experiment workflow you are using at /WesternWorkflowOverview. Molecular weights for Chameleon Pre-Stained Protein ladders are preset in Empiria Studio Software, allowing you to estimate band size without entering the molecular weights. The Chameleon Pre-stained Protein Ladders are not standards, and protein band migration may appear different depending on the gel type used. For individual molecular weights, please refer to the image above. The ladder can be used for easy visualization or two-color near-infrared detection. Do not heat above room temperature.ĭuring electrophoresis, the ladder will separate into 11 visible and near-infrared bands in the 8 - 260 kDa range. Mix the product thoroughly before use to dissolve any solids that may have precipitated during storage. Bring the product to room temperature while protecting from light. To see a complete portfolio of LI-COR molecular weight markers, visit /proteinmarkers.Ĭhameleon Pre-stained Protein Ladders are provided ready to use in loading buffer.

#Spec protein ladders for western blotting serial#

Two-fold serial dilutions of AzureSpectra-800 labeled antibody. It provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. The ladder is optimized for use with Bis-Tris and Tris-Glycine systems. The Azure 600 offers laser technology with two IR detection channels enabling you to image more than one protein in an assay. Red and green represent the 700 and 800 nm channels, respectively. The blot was scanned on an Odyssey CLx Imager. The example shows a visible image (left) and a two-color, near-infrared image (right) resolved on a 4 - 12% Bis-Tris Gel and transferred to nitrocellulose via wet tank transfer. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest. For a multi-lane gel load 5-50 g of protein per lane. In gels, the ladder can be used to visualize progress of the protein separation during electrophoresis and to estimate the molecular weight of unknown proteins based on their relative mobility. Using gel loading pipette tips, load cooled samples and protein marker onto gel. The Chameleon Duo Pre-stained Protein Ladder provides multicolored, pre-stained, and near-infrared fluorescent bands of convenient and consistent protein sizes.